In the world of cell biology, some tools stand the test of time. One of them is Medium 199 (M199) — a formulation that dates back to 1950 but remains a go-to choice for researchers, biologists, and vaccine developers even today.
Celhappy M199, now available at the CoPure, carries forward this legacy by offering a high-quality version of this versatile medium, optimized for a wide range of primary cell cultures and viral research applications.
A Medium with Historical Significance
Originally formulated by Morgan et al. for studying chicken embryo fibroblasts, M199 was one of the first media to be chemically defined. Its composition was meticulously designed to support the nutritional needs of complex cells — especially non-transformed primary cells, which often require a more refined and complete nutrient profile.
Over the decades, M199 has become a staple in:
Virology research
Vaccine production
Primary explant cultures, such as mouse pancreatic epithelium and rat lens tissue
Nutritional studies and metabolic assays
What Sets M199 Apart?
Compared to standard basal media like DMEM or RPMI, M199 stands out for its unique blend of purines, pyrimidines, and vitamins, such as:
Adenine
Adenosine
Hypoxanthine
Thymine
These components are essential for nucleic acid synthesis and cellular replication — particularly critical for slow-growing or sensitive cell types.
M199 also features balanced salt formulations suited to specific incubator conditions:
Earle’s salts – Ideal for CO₂ incubators
Hank’s salts – Suitable for ambient air (non-CO₂ environments)
(Celhappy M199 uses Earle’s salts by default, perfect for typical CO₂ lab conditions.)
Celhappy M199 – Product Details
| Feature |
Dry Powder |
Liquid |
| Glucose |
1.00 g/L |
1.00 g/L |
| HEPES Buffer |
Not included |
Not included |
| Sodium Pyruvate |
Not included |
Not included |
| Phenol Red (pH Indicator) |
Included |
Included |
| L-Glutamine |
Included |
Included |
| Sodium Bicarbonate |
Included |
Included |
| Balanced Salt Type |
Earle’s Salt |
Earle’s Salt |
| Sterilization |
Non-sterile |
Aseptic filtered |
| Storage Temp. |
2–8°C (protect from light) |
2–8°C (protect from light) |
| Shelf Life |
24 months |
12 months |
Key Benefits of Using Celhappy M199
Reliable Support for Primary Cells: Designed to meet the complex nutrient needs of delicate, non-transformed cell types.
Versatile Applications: Used widely in vaccine R&D, virology, organ explant cultures, and more.
High-Quality Manufacturing: Aseptic filtration and strict quality controls ensure consistency batch after batch.
Convenient Formats: Available in both dry powder (longer shelf life) and ready-to-use liquid (time-saving).
Looking for the Right Medium?
If you’re comparing media for a specific protocol, be sure to consult the M199 medium formula comparison chart. It provides a quick breakdown of key components like glucose, sodium pyruvate, HEPES, phenol red, and L-glutamine — helping you select the most compatible medium for your experiment.
Order Celhappy M199 Today from CoPure
Whether you’re culturing primary cells, scaling vaccine production, or running virology assays, Celhappy M199 is a proven choice backed by decades of scientific trust.
Available now at the CoPure in both liquid and powder formats.
For Further Enquiry Contact- sales@copure.com.au
