Preparing reliable, high-quality cell culture at scale is one of the hardest — and most mission-critical — parts of upstream bioprocessing. Lifecask™ RG single-use bioreactor bags from Cobetter (available via Copure) are engineered to make that step faster, safer and more predictable: they cover 50 L → 2,000 L, are built from Cobetter’s Lifemeta™ materials, and — crucially — have been validated with mixing, kLa and CFD work that supports real scale-up decisions.

The problem: scale, sterility and variability

When teams move from bench to pilot and then to manufacturing, three recurring friction points appear: (1) oxygen and mass-transfer performance that changes with scale, (2) long equipment changeover and validation cycles driven by CIP/SIP, and (3) process variability from manual interventions. These issues cost time, add risk of contamination and slow timelines for process development and commercialisation. Market adoption data show why manufacturers invest in single-use systems: the single-use bioreactor market is growing strongly as companies prioritise flexibility and faster time-to-clinic.

What Lifecask™ RG brings to the bench and the plant

Lifecask RG is designed as a practical, validated single-use option for suspension and adherent culture (CHO, HEK293, BHK21, hybridomas, Vero, hMSC, CGT workflows and more). Key technical strengths:

Scale coverage and geometry — sizes: 50, 200, 500, 1,000 and 2,000 L (turn-down ratios and H/D aspect ratios are engineered to maintain geometric similarity across scales).
Oxygenation — a patented split aeration disk plus bottom-mounted impeller geometry keeps oxygen transfer and mixing consistent across sizes.
Materials & low extractables — the bags use Cobetter’s Lifemeta™ KA film and Lifemeta™ tubing (STT silicone, STF TPE), with validation data for low extractables and endotoxin limits (aqueous extraction < 0.25 EU/mL by LAL).
Process coverage — multiple ports for pH/DO, probe sleeves, sampling bags, single-use pressure sensors and outlet filter ports to reduce leakage/contamination risk.

Data-driven validation — why that matters

Cobetter reports full performance validation, including mixing time measurements, kLa modelling, Newton-Power analysis, and CFD fluid studies to support scale-up claims. Those validation steps are exactly what process engineers rely on when transferring a process from a small stirred tank to a single-use vessel of 1,000 L or more. In practice, mixing time and kLa are the primary knobs used to ensure similar oxygen and nutrient delivery across scales — industry guidance recommends characterising power input (P/V), mixing time and kLa as part of any scale strategy. Typical kLa targets for cell culture systems are often in the low single-digit to low double-digit h⁻¹ range, depending on cell line and process; validated kLa/mixing data give you the numbers you need to match process performance.

Operational advantages vs. traditional stainless steel

Switching to single-use bags like Lifecask RG removes many time-consuming steps (no complex SIP/CIP cycles inside the vessel), reduces water and chemical consumption, and shortens changeover/turnaround. That lowers the risk of operator-introduced contamination and lets teams reallocate skilled labour to higher-value tasks (development, analytics, troubleshooting). Thermo Fisher and other industry reviews highlight these operational and sustainability benefits when comparing single-use to stainless systems.

Safety, quality and supply continuity

Cobetter manufactures the core components in-house (film, tubing, capsule filters, sampling bags, and probe sleeves) and runs the products through integrity and extractables testing. Documentation, such as COAs, validation guides, and sterilisation verification (gamma/ISO 11137 checks), is available for regulated environments. That level of vertical integration simplifies vendor qualification and supports audit trails for GMP production.

When Lifecask RG is the right choice

You need rapid tech-transfer from bench to pilot with minimal re-engineering.

Your process benefits from short changeover times, and you want to reduce CIP/SIP cycles and associated resource use.

You require documented validation (mixing, kLa, CFD) so that scale-up risks are quantified, rather than estimated.

Bottom line

Lifecask™ RG single-use bioreactor bags are a pragmatic, validated route to faster, lower-risk upstream processing. Their combination of geometric design, validated mass-transfer performance, low-extractable materials and operational safety features (pressure sensors, filter ports, integrity testing) gives process teams the data and predictability they need to push from R&D into commercial production with confidence. As market demand for flexible, single-use solutions continues its steep rise, proven components and documented validation will be what separates a risky scale-up from a reproducible, audit-ready process.
For Further Enquiry Contact- sales@copure.com.au